Immunologic studies using antigen-coated charcoal: Application to rheumatoid factor (RF)

Laboratory application of the dextran-coated-charcoal radioimmunoassay of insulin.

A modification of the method for radioimmunoassayof insulin is described, which achievessatisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution fo...

Immunologic studies with intrinsic factor in man.

Since the fundamental defect in pernicious anemia is inadequate or absent secretion of gastric intrinsic factor (1), specific therapy with intrinsic factor would seem, logically, to be the preferred treatment. After the demonstration (2, 3) that desiccated hog stomach could substitute for human gastric juice to facilitate the absorption of vitamin B12 in man, many patients with pernicious anemi...

Immunologic studies of antihemophilic factor (AHF, factor VIII). V. Immunologic properties of AHF subunits produced by salt dissociation.

Studies on the isolation of rheumatoid factor.

The need for the isolation of rheumatoid factor in a high state of purity and in sufficient quantity for antiserum production has led to an appraisal of the various purification methods available. Ziff, Brown, Lospalluto, Badin, and McEwen (1956) demonstrated that the sensitized sheep cell agglutinating activity of rheumatoid serum was precipitated in the euglobulin fraction, and Svartz and Sch...

Chromatographic Studies of the Rheumatoid Factor

Serum and serum fractions from patients with rheumatoid arthritis have been subjected to anion exchange chromatography on diethylaminoethyl cellulose. The rheumatoid factor activity was clearly separated from the bulk of the gamma globulin by virtue of the anomalous behavior of macroglobulins on this column. Purification of rheumatoid factor by precipitation with fraction II, resolution in 4 M ...